anti‑klb  (R&D Systems)

Journal: Diabetology & Metabolic Syndrome

Article Title: Effects of exercise intensity and diet on cardiac tissue structure and FGF21/β-Klotho signaling in type 2 diabetic mice: a comparative study of HFD and HFD + STZ induced type 2 diabetes models in mice

doi: 10.1186/s13098-024-01541-3

Figure Lengend Snippet: The two-way analysis of variance model concerning a diabetes-inducing model and ET

Article Snippet: The membranes were then blocked with 5% BSA (Cat No: A-7888; Sigma Aldrich, MO, USA) in 0.1% Tween 20 for 1 h. Then, the membranes were incubated with anti-beta Klotho (Cat No: LS-B3568, LifeSpan BioSciences, USA) and anti-β actin-loading control antibodies (Cat No: ab8227, Abcam, UK) for 1 h at room temperature.

Techniques:

Journal: Diabetology & Metabolic Syndrome

Article Title: Effects of exercise intensity and diet on cardiac tissue structure and FGF21/β-Klotho signaling in type 2 diabetic mice: a comparative study of HFD and HFD + STZ induced type 2 diabetes models in mice

doi: 10.1186/s13098-024-01541-3

Figure Lengend Snippet: a Changes in the serum levels of FGF21 in the control (C), high-intensity interval training (HIIT) and moderate-intensity continuous training (MICT) subgroups of the normal diet (ND), high-fat diet (HFD) and HFD with STZ injection (HFD + STZ) groups. Comparisons were made among the control subgroups of the ND, HFD and HFD + STZ groups to analyze the effect of the mouse model on the serum level of FGF21. Additionally, to analyze the effect of both training protocols on the serum levels of FGF21, the serum levels in the HIIT and MICT subgroups were compared with those in their control subgroups. b Changes in heart tissue protein levels of β-Klotho in the C, HIITMICT ND, HFD HFD + STZ groups. Comparisons were made among the control subgroups of the ND, HFD and HFD + STZ groups to analyze the effect of the mouse model on the heart tissue protein level of β-Klotho. The data are presented as the means. *p < 0.05, **p < 0.001 and # p = 0.053. The P value was calculated using Tukey’s post hoc test. c Western blot analysis of β-Klotho in heart tissue showing decreased expression of β-Klotho in HFD-C and HFD + STZ-C mice compared to that in ND-C mice. Additionally, an increase in β-Klotho expression was observed in HFD-HIIT and HFD-MICT mice compared to HFD-C mice, but the difference was not statistically significant

Article Snippet: The membranes were then blocked with 5% BSA (Cat No: A-7888; Sigma Aldrich, MO, USA) in 0.1% Tween 20 for 1 h. Then, the membranes were incubated with anti-beta Klotho (Cat No: LS-B3568, LifeSpan BioSciences, USA) and anti-β actin-loading control antibodies (Cat No: ab8227, Abcam, UK) for 1 h at room temperature.

Techniques: Control, Injection, Western Blot, Expressing

Journal: Diabetology & Metabolic Syndrome

Article Title: Effects of exercise intensity and diet on cardiac tissue structure and FGF21/β-Klotho signaling in type 2 diabetic mice: a comparative study of HFD and HFD + STZ induced type 2 diabetes models in mice

doi: 10.1186/s13098-024-01541-3

Figure Lengend Snippet: Linear relationship. a There was a significant inverse relationship between changes in fasting blood glucose (FBG) and changes in heart tissue protein levels of β-Klotho in the control subgroups of the ND, HFD and HFD + STZ groups (n = 12). b There was a significant direct relationship between changes in FBG and changes in the serum level of FGF21 in the control subgroups of the ND, HFD and HFD + STZ groups (n = 12). c There was a significant direct relationship between changes in FBG and changes in the serum level of FGF21 in the C, HIIT and MICT subgroups of HFD mice (n = 12). d There was a significant direct relationship between changes in FBG and changes in the serum level of FGF21 in the C, HIIT and MICT subgroups of HFD + STZ mice (n = 12). e There was a significant inverse relationship between changes in FBG and changes in heart tissue protein levels of β-Klotho in the C, HIIT and MICT subgroups of HFD + STZ mice (n = 12). “r” was calculated by Pearson correlation

Article Snippet: The membranes were then blocked with 5% BSA (Cat No: A-7888; Sigma Aldrich, MO, USA) in 0.1% Tween 20 for 1 h. Then, the membranes were incubated with anti-beta Klotho (Cat No: LS-B3568, LifeSpan BioSciences, USA) and anti-β actin-loading control antibodies (Cat No: ab8227, Abcam, UK) for 1 h at room temperature.

Techniques: Control